6RWT
Crystal structure of the Cbp3 homolog from Brucella abortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.418, 55.909, 97.585 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.420 - 1.420 |
| R-factor | 0.17004 |
| Rwork | 0.168 |
| R-free | 0.20127 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.180 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | Arcimboldo |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.400 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.052 | 1.906 |
| Rmeas | 0.054 | 1.992 |
| Rpim | 0.014 | 0.575 |
| Number of reflections | 46643 | 2242 |
| <I/σ(I)> | 18 | 1.2 |
| Completeness [%] | 99.9 | 98 |
| Redundancy | 13.5 | 11.5 |
| CC(1/2) | 1.000 | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 20% v/v polyethylene glycol 8000, 0.1 M TRIS pH 8.0, 0.2 M magnesium chloride hexahydrate |
