6RWT

Crystal structure of the Cbp3 homolog from Brucella abortus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9282
Spacegroup name	P 21 21 21
Unit cell lengths	44.418, 55.909, 97.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.420 - 1.420
R-factor	0.17004
Rwork	0.168
R-free	0.20127
Structure solution method	AB INITIO PHASING
RMSD bond length	0.009
RMSD bond angle	1.180
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	Arcimboldo
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.400	1.440
High resolution limit [Å]	1.420	1.420
Rmerge	0.052	1.906
Rmeas	0.054	1.992
Rpim	0.014	0.575
Number of reflections	46643	2242
<I/σ(I)>	18	1.2
Completeness [%]	99.9	98
Redundancy	13.5	11.5
CC(1/2)	1.000	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	20% v/v polyethylene glycol 8000, 0.1 M TRIS pH 8.0, 0.2 M magnesium chloride hexahydrate