6RWF
The dissociation mechanism of processive cellulases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.96700 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.363, 51.632, 65.590 |
| Unit cell angles | 90.00, 96.31, 90.00 |
Refinement procedure
| Resolution | 29.520 - 1.640 |
| R-factor | 0.1288 |
| Rwork | 0.127 |
| R-free | 0.16480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cel |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.732 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.480 | 1.680 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 44198 | 2624 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 97.5 | 91.1 |
| Redundancy | 1.6 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10 mM sodium acetate, pH 5.0, 20% PEG 5000 monomethyl ether, 0.1 M MES pH 6.0, 10 mM cobalt chloride, 12.5% glycerol. |
