6RVM

Cell division protein FtsZ from Staphylococcus aureus, apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	72.564, 78.805, 225.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.161 - 2.155
R-factor	0.2308
Rwork	0.230
R-free	0.25580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wgj
RMSD bond length	0.002
RMSD bond angle	0.454
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.270
High resolution limit [Å]	2.150	2.150
Number of reflections	70836	10226
<I/σ(I)>	13.6
Completeness [%]	99.9
Redundancy	5.5
CC(1/2)	0.998	0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.1 M (NH4)2SO4, 0.1 M MES pH 6.2