6RV0
human Alanine:Glyoxylate Aminotransferase major allele (AGT-Ma); with PMP in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.306, 90.306, 142.071 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.490 - 2.700 |
| R-factor | 0.2623 |
| Rwork | 0.260 |
| R-free | 0.32530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f9s |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.463 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.490 | 47.490 | 2.830 |
| High resolution limit [Å] | 2.700 | 8.960 | 2.700 |
| Rmerge | 0.069 | 0.029 | 1.120 |
| Rmeas | 0.074 | 0.031 | 1.193 |
| Rpim | 0.025 | 0.011 | 0.402 |
| Total number of observations | 141538 | 3868 | 18516 |
| Number of reflections | 16712 | 524 | 2202 |
| <I/σ(I)> | 15.8 | 49.4 | 1.9 |
| Completeness [%] | 99.5 | 97 | 100 |
| Redundancy | 8.5 | 7.4 | 8.4 |
| CC(1/2) | 0.999 | 0.999 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 10-12% PEG 6K; 5% 2-methyl-2,4-pentanediol (MPD); 0.1M MES pH 6.5 |
