6RUE

Wolinella succinogenes L-asparaginase mutant V23Q,K24T with L-Asp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.8
Spacegroup name	P 1 21 1
Unit cell lengths	64.920, 134.360, 73.150
Unit cell angles	90.00, 93.70, 90.00

Refinement procedure

Resolution	30.000 - 1.650
R-factor	0.17094
Rwork	0.169
R-free	0.19967
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.768
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.740
High resolution limit [Å]	1.650	1.650
Number of reflections	146129	21413
<I/σ(I)>	5.4172
Completeness [%]	97.6
Redundancy	2.89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION		293	PEG