6RUD

WOLINELLA SUCCINOGENES L-ASPARAGINASE P1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.8
Spacegroup name	P 1 21 1
Unit cell lengths	62.820, 109.170, 87.700
Unit cell angles	90.00, 95.50, 90.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.16207
Rwork	0.160
R-free	0.19386
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.747
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.180	1.790
High resolution limit [Å]	1.700	1.700
Number of reflections	125360	18244
<I/σ(I)>	9.2205
Completeness [%]	97.2
Redundancy	2.91

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION		293	PEG