6RUD
WOLINELLA SUCCINOGENES L-ASPARAGINASE P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.820, 109.170, 87.700 |
| Unit cell angles | 90.00, 95.50, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.16207 |
| Rwork | 0.160 |
| R-free | 0.19386 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.747 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.180 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 125360 | 18244 |
| <I/σ(I)> | 9.2205 | |
| Completeness [%] | 97.2 | |
| Redundancy | 2.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 293 | PEG |
