6RU4

Structure of the SBP FpvC from pseudomonas aeruginosa in complex with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	44.010, 178.290, 66.820
Unit cell angles	90.00, 90.61, 90.00

Refinement procedure

Resolution	44.570 - 2.490
R-factor	0.198
Rwork	0.196
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r3z
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.570	2.640
High resolution limit [Å]	2.490	2.490
Rpim	0.070
Number of reflections	35191	5188
<I/σ(I)>	8.58
Completeness [%]	98.4
Redundancy	6.9
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	MPEG 2K, 0.1 M MES