6RU0
Light-Regulation of Imidazole Glycerol Phosphate Synthase by Interference with its Allosteric Machinery through Photo-Sensitive Unnatural Amino Acids
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-24 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1 |
Spacegroup name | P 32 |
Unit cell lengths | 95.415, 95.415, 165.930 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.964 - 2.648 |
R-factor | 0.2104 |
Rwork | 0.207 |
R-free | 0.27210 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.152 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.960 | 2.743 |
High resolution limit [Å] | 2.648 | 2.648 |
Rmerge | 0.105 | 1.782 |
Rmeas | 0.110 | 1.881 |
Rpim | 0.034 | 0.598 |
Number of reflections | 49035 | 4795 |
<I/σ(I)> | 17.15 | 1.1 |
Completeness [%] | 99.7 | 98.06 |
Redundancy | 10.4 | 9.6 |
CC(1/2) | 0.999 | 0.378 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG |