6RTT
Piperideine-6-carboxylate dehydrogenase from Streptomyces clavuligerus complexed with picolinic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.820, 130.060, 157.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.330 - 1.650 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rtr |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.330 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmeas | 0.088 | 0.706 |
| Number of reflections | 128777 | 5590 |
| <I/σ(I)> | 18.65 | 3.29 |
| Completeness [%] | 99.0 | 92 |
| Redundancy | 7.4 | 7.2 |
| CC(1/2) | 0.999 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M sodium acetate pH 5, 2.2 M ammonium sulphate, 0.2 M lithium acetate |
