6RTG
Crystal structure of the UDP-bound glycosyltransferase domain from the YGT toxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-15 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 164.270, 164.270, 47.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.560 - 1.900 |
| R-factor | 0.187 |
| Rwork | 0.186 |
| R-free | 0.22250 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.640 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.184 | 2.083 |
| Number of reflections | 52064 | 7314 |
| <I/σ(I)> | 12.07 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.4 | 19.2 |
| CC(1/2) | 0.996 | 0.525 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 277 | 100 mM Tris pH 8.5, 200 mM sodium acetate and 15 % PEG 4000 |
