6RT3
Native tetragonal lysozyme - synchrotron data
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.863, 78.863, 36.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.224 - 1.050 |
| R-factor | 0.1269 |
| Rwork | 0.126 |
| R-free | 0.14720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w1x |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.820 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.430 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.061 | |
| Rmeas | 0.064 | |
| Rpim | 0.017 | |
| Number of reflections | 53373 | 1955 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 97.6 | 73.8 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Sodium Acetate pH 4.8, 10% (w/v) NaCl, 25% (v/v) Ethylene Glycol |
