6RSW

HFD domain of mouse CAP1 bound to the pointed end of G-actin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	87.370, 54.490, 87.830
Unit cell angles	90.00, 93.61, 90.00

Refinement procedure

Resolution	41.450 - 1.950
R-factor	0.168
Rwork	0.166
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3daw 1s0p
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	XDS (VERSION Jan 26, 2018 BUILT=20180409)
Data scaling software	XSCALE (VERSION Jan 26, 2018 BUILT=20180409)
Phasing software	BALBES (1.0.0)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.450	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.088	1.073
Rmeas	0.096	1.174
Rpim	0.038	0.470
Number of reflections	60448	1209
<I/σ(I)>	12.88	1.58
Completeness [%]	99.9	99.87
Redundancy	6.4	6.2
CC(1/2)	0.999	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1M HEPES, 0.1 mM KCl, 10% PEG4000 (w/v)