6RSN

SOSEKI polymerising domain (SOK4 D85A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 61
Unit cell lengths	47.659, 47.659, 67.971
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.280 - 1.700
R-factor	0.1861
Rwork	0.185
R-free	0.21450
Structure solution method	SAD
Starting model (for MR)	model
RMSD bond length	0.012
RMSD bond angle	2.177
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0256)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.280	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.075	0.660
Rmeas	0.077	0.677
Rpim	0.017	0.149
Number of reflections	9612	949
<I/σ(I)>	33.91	5.81
Completeness [%]	99.5	99.06
Redundancy	20	20.2
CC(1/2)	1.000	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.69 M ammonium sulfate, 200 mM NaCl, 100 mM HEPES pH 7.0