6RSL
Cytochrome c co-crystallized with 10 eq. sulfonato-calix[8]arene and 25 eq. spermine (dry-coating method) - structure III
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98009 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 105.036, 105.036, 86.622 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.383 - 1.988 |
R-factor | 0.2425 |
Rwork | 0.241 |
R-free | 0.26470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gd9 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.392 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.272 | 2.022 |
High resolution limit [Å] | 1.988 | 1.988 |
Rmerge | 0.344 | 2.169 |
Rmeas | 0.351 | |
Rpim | 0.072 | 0.428 |
Number of reflections | 33926 | 1668 |
<I/σ(I)> | 6.4 | |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 25.5 | 26.7 |
CC(1/2) | 0.995 | 0.928 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.10 M HEPES pH 7.5, 0.15 M NaCl, 2.20 M Ammonium sulfate, 0.02 M sulfonato-calix[8]arene, 0.05 M spermine |