6RR3
CRYSTAL STRUCTURE OF FAD-CONTAINING FERREDOXIN-NADP REDUCTASE FROM BRUCELLA OVIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.968620 |
| Spacegroup name | P 41 |
| Unit cell lengths | 39.068, 39.068, 164.911 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.230 - 1.690 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qdx |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.230 | 1.780 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.059 | 0.347 |
| Number of reflections | 26843 | 3432 |
| <I/σ(I)> | 12.2 | 2 |
| Completeness [%] | 97.4 | 85.1 |
| Redundancy | 4.1 | 2 |
| CC(1/2) | 0.998 | 0.865 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 25% PEG 4K, 0.1 M MES pH 6.5 and 0.2 mM MgCl2 |
