6RQO
Steady-state-SMX activated state structure of bacteriorhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2018-06-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 62.000, 62.000, 110.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.336 - 2.000 |
| R-factor | 0.2074 |
| Rwork | 0.205 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b6z |
| Data reduction software | CrystFEL (0.7) |
| Data scaling software | CrystFEL (0.7) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.400 | 2.100 | 1.850 |
| High resolution limit [Å] | 2.000 | 2.000 | 1.800 |
| Number of reflections | 15903 | 2127 | |
| <I/σ(I)> | 1 | 0.36 | |
| Completeness [%] | 98.1 | 99 | 99.5 |
| Redundancy | 1 | 1 | 58.1 |
| CC(1/2) | 0.050 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.6 | 294 | 100 mM Na/K Phosphate buffer pH 5.6 30 % PEG 2000 |
| 1 | LIPIDIC CUBIC PHASE | 5.6 | 294 | 100 mM Na/K Phosphate buffer pH 5.6 30 % PEG 2000 |
| 1 | LIPIDIC CUBIC PHASE | 5.6 | 294 | 100 mM Na/K Phosphate buffer pH 5.6 30 % PEG 2000 |
