6RQM

A blocking anti-CTLA-4 Nanobody (KN044) complexed with CTLA-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 65 2 2
Unit cell lengths	98.770, 98.770, 110.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.730 - 3.000
R-factor	0.25272
Rwork	0.250
R-free	0.31258
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jds 3osk
RMSD bond length	0.002
RMSD bond angle	1.206
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.000	3.180
High resolution limit [Å]	3.000	3.000
Rmerge	0.110	1.080
Rmeas	0.121
Rpim	0.032
Number of reflections	6820	1068
<I/σ(I)>	18.8	3
Completeness [%]	100.0	100
Redundancy	14	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG400,