6RQG

P46, an immunodominant surface protein from Mycoplasma hyopneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	136.478, 136.478, 139.132
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.390 - 3.100
R-factor	0.2057
Rwork	0.203
R-free	0.24860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ywh 3urm 4wwh
RMSD bond length	0.003
RMSD bond angle	0.649
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.900	3.200
High resolution limit [Å]	3.100	3.100
Number of reflections	26816	4774
<I/σ(I)>	22.88
Completeness [%]	97.6
Redundancy	10.56	1.9
CC(1/2)	0.970	0.170

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1 M Tris pH 8.5 0.2 M Sodium Acetate 32% PEG 4000