6RPH

TR-SMX open state structure (10-15ms) of bacteriorhodopsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	298
Detector technology	PIXEL
Collection date	2018-07-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 63
Unit cell lengths	62.000, 62.000, 110.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.000 - 2.600
R-factor	0.3202
Rwork	0.317
R-free	0.35990
RMSD bond length	0.002
RMSD bond angle	0.368
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.000	2.980	2.180
High resolution limit [Å]	2.600	2.600	2.100
Number of reflections	6477	1838
<I/σ(I)>	1		1.33
Completeness [%]	87.3	80	100
Redundancy	1	1	24.6
CC(1/2)			0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.6	294	100 mM Na/K Phosphate buffer pH 5.6 30 % PEG 2000
1	LIPIDIC CUBIC PHASE	5.6	294	100 mM Na/K Phosphate buffer pH 5.6 30 % PEG 2000
1	LIPIDIC CUBIC PHASE	5.6	294	100 mM Na/K Phosphate buffer pH 5.6 30 % PEG 2000