6ROL

Structure of IMP2 KH34 domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 18-ID
Synchrotron site	APS
Beamline	18-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	76.880, 62.380, 85.740
Unit cell angles	90.00, 91.32, 90.00

Refinement procedure

Resolution	37.800 - 2.100
Rwork	0.198
R-free	0.22380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3krm
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.800	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.057	0.411
Rmeas	0.080	0.582
Rpim	0.057	0.411
Number of reflections	46202	4632
<I/σ(I)>	8.05	2.05
Completeness [%]	96.9	98.74
Redundancy	1.9	1.9
CC(1/2)	0.994	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	23% PEG-3350, 200mM Ammonium sulfate, 100mM Tris pH 8.0