6RO6

Crystal structure of the C-terminal dimerization domain of the essential repressor DdrO from radiation-resistant Deinococcus bacteria (Deinococcus deserti)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-18
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9660
Spacegroup name	P 21 21 21
Unit cell lengths	51.910, 76.800, 102.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 - 1.410
R-factor	0.1962
Rwork	0.195
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rnx
RMSD bond length	0.018
RMSD bond angle	1.533
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.420	1.430
High resolution limit [Å]	1.410	1.410
Rmerge	0.037
Number of reflections	73609	3911
<I/σ(I)>	17.5
Completeness [%]	92.4
Redundancy	4.9
CC(1/2)		0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium Citrate Tribasic pH 4.5, 0.2 M Lithium sulfate, 46% w/v PEG 400