6RN1

Structure of N-terminal truncated Plasmodium falciparum IMP-nucleotidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-18
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.967
Spacegroup name	P 42 21 2
Unit cell lengths	140.660, 140.660, 86.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.578 - 3.000
R-factor	0.2536
Rwork	0.252
R-free	0.27980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rmd
RMSD bond length	0.002
RMSD bond angle	0.497
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.580	3.300
High resolution limit [Å]	3.000	3.000
Number of reflections	18036	4376
<I/σ(I)>	20.8
Completeness [%]	99.8
Redundancy	27
CC(1/2)	0.960	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.06M citric acid, 0.04M Bis-tris propane pH 4.1 and 16% PEG 3350