6RMT

Crystal structure of disulphide-linked human C3d dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	54.624, 59.896, 175.488
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.685 - 2.000
R-factor	0.1451
Rwork	0.142
R-free	0.19960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wy7
RMSD bond length	0.008
RMSD bond angle	0.948
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.500	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.177	1.002
Rpim	0.038	0.223
Number of reflections	39938	2880
<I/σ(I)>	14.1	4.6
Completeness [%]	100.0	99.7
Redundancy	21.1	21.1
CC(1/2)	0.998	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	100 mM Tris pH 8, 200 mM NaCl, 24% PEG 4000