6RMR
Crystal structure of Escherichia coli periplasmic glucose-1-phosphatase H18D mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9340 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.035, 101.651, 114.929 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.768 - 2.500 |
R-factor | 0.228870454809 |
Rwork | 0.225 |
R-free | 0.26615 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nt4 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.617 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.483 | 2.589 |
High resolution limit [Å] | 2.300 | 2.500 |
Rmerge | 0.139 | 1.257 |
Rmeas | 0.152 | 1.361 |
Rpim | 0.059 | 0.517 |
Number of reflections | 26470 | 2595 |
<I/σ(I)> | 10.39 | |
Completeness [%] | 99.2 | |
Redundancy | 6.5 | |
CC(1/2) | 0.997 | 0.637 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 0.1 M CHES, pH 9.5, 20 % w/v PEG 800 13 mg/mL AGP in 10 mM NaAc p 4.5 |