6RMK

Bacteriorhodopsin, dark state, cell 2, refined using the same protocol as sub-ps time delays

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE CXI
Synchrotron site	SLAC LCLS
Beamline	CXI
Temperature [K]	293
Detector technology	PIXEL
Collection date	2017-08-06
Detector	CS-PAD CXI-2
Wavelength(s)	1.26
Spacegroup name	P 63
Unit cell lengths	62.100, 62.100, 110.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.441 - 1.800
R-factor	0.2439
Rwork	0.243
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b6v
RMSD bond length	0.006
RMSD bond angle	0.933
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX ((dev_2645: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.444	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	22403	1140
<I/σ(I)>	9.9
Completeness [%]	100.0
Redundancy	476

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	32% (W/V) PEG 2000, 0.1 M K2HPO4 /NAH2PO4, IN HAMILTON SYRINGES, PH 5.6, LIPIDIC CUBIC PHASE,