6RMC

Crystal structure of the DEAH-box ATPase Prp2 in complex with Spp2 and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	74.730, 113.690, 191.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.896 - 2.600
R-factor	0.2235
Rwork	0.222
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4YB2
RMSD bond length	0.004
RMSD bond angle	0.539
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.710
High resolution limit [Å]	2.600	2.600
Rmerge	0.069	0.930
Rmeas	0.077
Total number of observations	256605
Number of reflections	50939	6069
<I/σ(I)>	17.33	1.83
Completeness [%]	99.6	99.7
Redundancy	5.04
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	100 mM Bicine/Trizma pH 7.5, 11% (w/v) PEG8000, 20% (w/v) ethylene glycol, 20 mM of a monosaccharide mixture (D-glucose, D-mannose, D-galactose, L-fucose, D-xylose and N-acetyl-D-glucosamine)