6RMB

Crystal structure of the DEAH-box ATPase Prp2 in complex with Spp2 and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97620
Spacegroup name	P 1 21 1
Unit cell lengths	69.360, 72.640, 70.270
Unit cell angles	90.00, 93.29, 90.00

Refinement procedure

Resolution	70.154 - 2.500
R-factor	0.2107
Rwork	0.208
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.154	70.154	2.600
High resolution limit [Å]	2.500	50.000	2.500
Rmerge	0.078		0.661
Rmeas	0.094		0.798
Total number of observations	75644
Number of reflections	23946	1	2667
<I/σ(I)>	12.16		1.92
Completeness [%]	98.5	20	98.7
Redundancy	3.159	1	3.072
CC(1/2)	0.997		0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	100 mM HEPES/NaOH pH 7, 8% (w/v) polyvinyl alcohol, 7.5% (v/v) isopropyl alcohol