6RM9

Crystal structure of the DEAH-box ATPase Prp2 in complex with Spp2 and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	65.850, 77.420, 148.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.689 - 1.850
R-factor	0.1986
Rwork	0.198
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fa5
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	68.689	68.689	1.950
High resolution limit [Å]	1.850	50.000	1.850
Rmerge	0.066	0.619	1.099
Rmeas	0.075	0.876	1.245
Total number of observations	298478
Number of reflections	65564	5	9432
<I/σ(I)>	11.45	4.86	1.84
Completeness [%]	99.7	100	99.8
Redundancy	4.552	1.4	4.531
CC(1/2)	0.998		0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	100 mM Bis-Tris pH 5.5, 400 mM ammonium sulfate, 23% (w/v) PEG3350