6RM8

Crystal structure of the DEAH-box ATPase Prp2 in complex with Spp2 and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	P 21 21 21
Unit cell lengths	64.340, 75.460, 154.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.526 - 1.950
R-factor	0.2009
Rwork	0.200
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fa5
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.526	42.526	2.050
High resolution limit [Å]	1.950	50.000	1.950
Rmerge	0.074		1.335
Rmeas	0.078		1.407
Total number of observations	520880
Number of reflections	55604	3	7614
<I/σ(I)>	15.97		2.08
Completeness [%]	100.0	75	99.9
Redundancy	9.368	2	9.753
CC(1/2)	0.999		0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	100 mM MES pH 6.5, 350 mM calcium acetate, 19% (w/v) PEG4000