6RKE

Molybdenum storage protein - P212121, ADP, molybdate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	128.060, 148.860, 183.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.492 - 1.700
R-factor	0.1947
Rwork	0.194
R-free	0.21370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ndo
RMSD bond length	0.006
RMSD bond angle	2.520
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.700	1.700
Number of reflections	378712	59518
<I/σ(I)>	13.2
Completeness [%]	99.2
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	2 mM landthanide, 0.1 M buffer system 4, ph 6.5, 30% precipitant mix 7