6RKA

Inter-dimeric interface controls function and stability of S-methionine adenosyltransferase from U. urealiticum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	I 41
Unit cell lengths	114.468, 114.468, 228.115
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.620 - 2.500
Rwork	0.233
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rjs
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.620	2.580
High resolution limit [Å]	2.500	2.500
Rmerge	0.309	0.610
Number of reflections	50487	4625
<I/σ(I)>	11
Completeness [%]	100.0
Redundancy	9.3
CC(1/2)	0.999	0.397

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2m Li2SO4, 0.1M Tris pH 8.5 and 25% PEG 3350