6RJS

Inter-dimeric interface controls function and stability of S-methionine adenosyltransferase from U. urealiticum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.976250
Spacegroup name	P 1
Unit cell lengths	56.205, 64.064, 114.495
Unit cell angles	77.93, 83.86, 73.97

Refinement procedure

Resolution	47.620 - 2.600
Rwork	0.212
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t8s
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	111.810	2.730
High resolution limit [Å]	2.600	2.600
Rmerge	0.098	0.648
Rpim	0.055
Number of reflections	42461	12471
<I/σ(I)>	12.4
Completeness [%]	92.0
Redundancy	4.1
CC(1/2)		0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	5% Tacsimate, 0.1M Hepes pH 7.0, 10% PEG 5K