6RJI

X-ray structure of the elongation factor P of S. aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	30.266, 43.460, 126.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.097 - 1.480
R-factor	0.1815
Rwork	0.179
R-free	0.22090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yby
RMSD bond length	0.007
RMSD bond angle	0.884
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.100	1.533
High resolution limit [Å]	1.480	1.480
Rmerge	0.046	0.751
Rmeas	0.050	0.811
Number of reflections	28702	2814
<I/σ(I)>	20.44	2.97
Completeness [%]	99.9	99.82
Redundancy	6.9	7.1
CC(1/2)	0.999	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.2M Imidazole malate 24% PEG600 pH 5.5