6RJC

E.coli transketolase apoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.8266
Spacegroup name	P 21 21 21
Unit cell lengths	89.905, 102.048, 132.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.947 - 1.050
R-factor	0.1042
Rwork	0.104
R-free	0.12330
RMSD bond length	0.006
RMSD bond angle	0.991
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.13_2998)
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.950	1.090
High resolution limit [Å]	1.050	1.050
Rmeas	0.055	0.818
Number of reflections	549750	57085
<I/σ(I)>	15.71	2.36
Completeness [%]	97.5	96.1
Redundancy	4.6	4.6
CC(1/2)	1.000	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	279.15	PEG6000, Glycerol, Glycyl-Glycine