6RJ1

N-Domain P40/P90 Mycoplasma pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-07-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97927
Spacegroup name	P 21 21 21
Unit cell lengths	97.796, 114.434, 165.054
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.530 - 2.650
R-factor	0.215
Rwork	0.214
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5MZB
RMSD bond length	0.008
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.800	2.790
High resolution limit [Å]	2.650	2.650
Number of reflections	54524	7879
<I/σ(I)>	8.6
Completeness [%]	99.8
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	PEG3350 and MetOH