6RIX
Crystal structure of MchDnaB-1 intein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.246, 95.344, 35.796 |
| Unit cell angles | 90.00, 107.14, 90.00 |
Refinement procedure
| Resolution | 47.672 - 1.655 |
| R-factor | 0.1612 |
| Rwork | 0.159 |
| R-free | 0.21370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bs8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.847 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.655 | 1.655 |
| Rmerge | 0.085 | 0.367 |
| Number of reflections | 22641 | 731 |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 95.0 | 60.2 |
| Redundancy | 6 | 2.7 |
| CC(1/2) | 0.993 | 0.543 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | Tris-HCl pH 9, magnesium chloride, polyethylene glycol 4000 |
