6RIV
Crystal structure of Alopecurus myosuroides GSTF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.289, 50.708, 51.603 |
| Unit cell angles | 97.74, 110.24, 110.96 |
Refinement procedure
| Resolution | 46.200 - 1.330 |
| R-factor | 0.1325 |
| Rwork | 0.131 |
| R-free | 0.16340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aw9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.541 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 1.350 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmeas | 0.054 | 0.693 |
| Rpim | 0.029 | |
| Number of reflections | 93011 | 1536 |
| <I/σ(I)> | 17 | 1.7 |
| Completeness [%] | 94.9 | 32 |
| Redundancy | 6.8 | 2.9 |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 289 | 0.1M SPG, pH 5; 25% w/v PEG 1500 |
