6RI3

Dodecin from Streptomyces davaonensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.07
Spacegroup name	P 43 21 2
Unit cell lengths	72.288, 72.288, 151.213
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.170 - 2.400
Rwork	0.220
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yiz
RMSD bond length	0.008
RMSD bond angle	1.448
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Number of reflections	16434	1694
<I/σ(I)>	28
Completeness [%]	100.0
Redundancy	11
CC(1/2)		0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M (NH4)2SO4, 10% (w/v) PEG-4000, 0.1 M NaOAc, pH 4.8