6RHS
Crystal structure of Pediococcus acidilactici (Putative)lactate oxidase Refolded WT protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 135.500, 135.500, 125.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.950 - 2.600 |
| R-factor | 0.18728 |
| Rwork | 0.186 |
| R-free | 0.21514 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6r9v |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.680 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 18301 | 1328 |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 1.1M Sodium Malonate, 0.1M Hepes pH 7.0, 0.5% jeffamine ED-2003. |
