6RHN

HISTIDINE TRIAD NUCLEOTIDE-BINDING PROTEIN (HINT) FROM RABBIT WITHOUT NUCLEOTIDE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-02
Detector	RIGAKU RAXIS II
Spacegroup name	P 43 21 2
Unit cell lengths	40.340, 40.340, 143.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.150
R-factor	0.176
Rwork	0.176
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PROTEIN PORTION OF HINT-ADENOSINE MODEL PDB ENTRY 4RHN
RMSD bond length	0.010
RMSD bond angle	24.900 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.140	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.046
Total number of observations	42366 *
Number of reflections	6302
Completeness [%]	90.0	59
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4 *		30% POLYETHYLENE GLYCOL 8000, 0.1 M SODIUM ACETATE, 0.1 M SODIUM CACODYLATE, PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG8000	30 (%)
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir	sodium cacodylate	0.1 (M)
5	1	drop	Tris-Cl	20 (mM)
6	1	drop		150 (mM)	pH7.4