6RH9

Crystal Structure of Two-Domain Laccase mutant I170F from Streptomyces griseoflavus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97242
Spacegroup name	P 1
Unit cell lengths	77.250, 94.770, 116.180
Unit cell angles	90.13, 89.95, 92.56

Refinement procedure

Resolution	47.337 - 1.850
R-factor	0.1814
Rwork	0.178
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o4q
RMSD bond length	0.008
RMSD bond angle	0.874
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.850	1.850
Rmerge		0.800
Rmeas	0.109
Number of reflections	256815	12984
<I/σ(I)>	10.9
Completeness [%]	91.4
Redundancy	2.08

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	296	25% Smear medium PEG, 0.1 M Bicine, 0.1 M NaCl, 0.05M H3BO3-NaOH