6RG1
Photorhabdus laumondii lectin PLL2 in complex with L-rhamnose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-23 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 65.066, 71.003, 85.764 |
| Unit cell angles | 90.00, 104.58, 90.00 |
Refinement procedure
| Resolution | 45.050 - 1.700 |
| R-factor | 0.1828 |
| Rwork | 0.181 |
| R-free | 0.20945 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.217 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | MOLREP (11.6.02) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.110 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.133 | 0.962 |
| Number of reflections | 41015 | 5990 |
| <I/σ(I)> | 4.4 | 1.3 |
| Completeness [%] | 98.6 | 98.8 |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.975 | 0.519 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 8% PEG 4000, 150 mM sodium acetate pH 4.6 |
