6RFG

Structure of the Vaccinia core protein E11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.007200
Spacegroup name	P 21 21 21
Unit cell lengths	75.560, 59.780, 138.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.381 - 1.897
R-factor	0.2073
Rwork	0.206
R-free	0.22880
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.632
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3247: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.000
High resolution limit [Å]	1.897	1.897
Number of reflections	48724	4328
<I/σ(I)>	11.1
Completeness [%]	96.6	87.4
Redundancy	6.4	6.1
CC(1/2)		0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	288	30% PEG4000 150 mM NaCl 5mM DTT