6RFA
Crystal structure of the H30K mutant of the light-driven sodium pump KR2 in the monomeric form, pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-2 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.968 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 40.738, 84.178, 234.541 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.890 - 2.200 |
| R-factor | 0.2107 |
| Rwork | 0.209 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xtl |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.729 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.090 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 21097 | 1825 |
| <I/σ(I)> | 7.4 | 1 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.4 | |
| CC(1/2) | 0.997 | 0.476 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 2.0 M Sodium Malonate, pH 8.0 |
