6RF9
Crystal structure of the Y154F mutant of the light-driven sodium pump KR2 in the monomeric form, pH 8.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.972 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 40.472, 81.851, 233.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.939 - 1.800 |
R-factor | 0.1653 |
Rwork | 0.163 |
R-free | 0.20340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xtl |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.3) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998: ???) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.930 | 40.930 | 1.840 |
High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
Rmerge | 0.061 | 0.040 | 0.870 |
Rmeas | 0.069 | 0.045 | 0.972 |
Rpim | 0.031 | 0.021 | 0.428 |
Total number of observations | 181740 | 1524 | 11212 |
Number of reflections | 36488 | 342 | 2168 |
<I/σ(I)> | 13.2 | 39.5 | 1.7 |
Completeness [%] | 99.6 | 98.1 | 99.6 |
Redundancy | 5 | 4.5 | 5.2 |
CC(1/2) | 0.998 | 0.996 | 0.885 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 2.0 M Sodium Malonate, pH 8.0 |