6RCC

Domain C P140 Mycoplasma genitalium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-06-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.07216
Spacegroup name	I 4 2 2
Unit cell lengths	90.090, 90.090, 58.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.040 - 1.430
R-factor	0.184
Rwork	0.183
R-free	0.19900
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXDE
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.040	1.510
High resolution limit [Å]	1.430	1.430
Number of reflections	20650
<I/σ(I)>	11.5
Completeness [%]	92.4
Redundancy	6.3
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	PEG3350, mgCl2 x 6H20 and TRIS buffer