6RBL
TETR(D) E147A MUTANT IN COMPLEX WITH DOXYCYCLINE AND MAGNESIUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-18 |
Detector | RIGAKU SATURN 70 |
Wavelength(s) | 1.5418 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 68.300, 68.300, 179.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.170 - 1.900 |
R-factor | 0.21242 |
Rwork | 0.209 |
R-free | 0.24640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fpl |
RMSD bond length | 0.012 |
RMSD bond angle | 1.772 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.170 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.099 | 0.371 |
Number of reflections | 17126 | 1617 |
<I/σ(I)> | 12.3 | 1.9 |
Completeness [%] | 99.4 | 98.2 |
Redundancy | 7.1 | 4.29 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Precipitant: 2.0M (NH4)2SO4, 3% PEG 400, 0.1M MES pH 6.5, Protein: 0.1mL protein, 0.1,L 2mM doxyTc, 0.001mL 3M MgCl2, 100mM NaCl, 50mM Tris pH8.0, 1/3 protein/precipitant |