6RBB

CRYSTAL STRUCTURE OF the VhH-domain of anti-IL-17A antibody netakimab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	P 2 21 21
Unit cell lengths	54.872, 72.499, 129.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.846 - 2.450
R-factor	0.2188
Rwork	0.215
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qkd
RMSD bond length	0.009
RMSD bond angle	1.053
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.520
High resolution limit [Å]	2.380	2.380
Rmeas	0.154
Number of reflections	21024	3076
<I/σ(I)>	9.1	1.4
Completeness [%]	98.3	90.7
Redundancy	5.1
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	296	50mMNa AcpH 5.5, 150mMNaCl with 0.1M Citrate pH 5, 20% (w/v) PEG 6000