6R9V
Crystal structure of Pediococcus acidilactici lactate oxidase A94G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 135.530, 135.530, 124.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.920 - 2.000 |
| R-factor | 0.15306 |
| Rwork | 0.152 |
| R-free | 0.17512 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j6x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.544 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 39425 | 2879 |
| <I/σ(I)> | 11.98 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 1.1M Sodium Malonate, 0.1M Hepes pH 7.0, 0.5% Jeffamine ED-2003. |
