6R9Q

Structure of Saccharomyces cerevisiae apo Pan2 pseudoubiquitin hydrolase-RNA exonuclease (UCH-Exo) module in complex with AACCAA RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	I 2 2 2
Unit cell lengths	92.189, 117.324, 256.658
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.488 - 3.079
R-factor	0.2625
Rwork	0.261
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4czw
Data reduction software	XDS
Data scaling software	xia2 (1.12)
Phasing software	PHASER (2.8)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.490	3.160
High resolution limit [Å]	3.079	3.079
Rmerge	0.086	1.733
Number of reflections	26217	11967
<I/σ(I)>	12.7	1.1
Completeness [%]	100.0	100
Redundancy	6.6	6.3
CC(1/2)	0.917	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.4 M ammonium phosphate